How to Remove Urine Stains and Smell from a Mattress?

Introduction

Urine stains and odors on a mattress can be frustrating and embarrassing—but the good news is, you can effectively remove both with the right method and a few common household items. Whether it’s from a child’s nighttime accident, an elderly family member, or even a pet, this guide will show you step-by-step how to tackle the problem quickly and thoroughly.

Did you know that urine not only stains the surface but also penetrates deep into the mattress layers, making odor removal tricky if not addressed immediately? That’s why acting fast is key—and why we’ve compiled a reliable system that truly works.

In this article, you’ll learn:

• How to remove fresh and old urine stains
• How to eliminate odors permanently—not just mask them
• What household products actually work and why
• When to use enzymatic cleaners for tough cases
• Bonus: The best tools and products to prevent future stains

Best Products for Removing Urine Stains and Odor from a Mattress

When tackling urine stains and odors, using the right products can make all the difference. Here are three top-rated options that consistently deliver results—backed by user reviews, expert endorsements, and real-world performance.

1. Rocco & Roxie Professional Strength Stain & Odor Eliminator

• Why we picked it: This enzymatic cleaner breaks down urine at the molecular level, eliminating both the stain and the smell—no masking scents or chemical after-smell.
• Key features:
  • Safe for pets and children
  • Certified by the Carpet and Rug Institute
  • Works on mattresses, carpets, and upholstery

2. Nature’s Miracle Urine Destroyer Plus

• Why we picked it: A powerful bio-enzymatic formula designed specifically for tough urine stains and deep odors, especially from pets.
• Key features:
  • Penetrates deeply into mattress layers
  • Light fresh scent—not overpowering
  • Trusted by pet owners for over 35 years

3. Bissell Little Green Portable Carpet & Upholstery Cleaner

• Why we picked it: Ideal for deep cleaning urine-soaked areas with a combination of suction, scrubbing, and professional cleaning formulas.
• Key features:
  • Portable and easy to use
  • Removes embedded stains and odors
  • Works well on mattresses, couches, and rugs

Step-by-Step Guide: How to Clean Fresh Urine from a Mattress

Acting quickly can make all the difference when it comes to removing fresh urine from a mattress. The faster you respond, the less chance the stain and smell have to settle deep into the foam or fabric layers.

Step 1: Blot, Don’t Rub

• Use paper towels or a clean, dry cloth to gently blot up as much liquid as possible.
• Avoid rubbing—it pushes urine deeper into the mattress.

Step 2: Apply a Vinegar Solution

• Mix a 50/50 solution of white vinegar and cold water in a spray bottle.
• Generously spray the stained area until it’s damp (not soaked).
• Let it sit for 10–15 minutes to help neutralize the odor and loosen the stain.

Step 3: Blot Again

• Use fresh towels to blot up the vinegar solution.
• Repeat until the mattress feels slightly damp but not wet.

Step 4: Sprinkle Baking Soda

• Liberally cover the area with baking soda.
• This draws out moisture and helps absorb odors.
• Leave it to sit for at least 8 hours (overnight is ideal).

Step 5: Vacuum Thoroughly

• Use a vacuum with an upholstery attachment to remove all the dried baking soda.
• Repeat if any odor or residue remains.

Quick Checklist:

• White vinegar
• Cold water
• Spray bottle
• Baking soda
• Towels & vacuum

 Avoid These Mistakes:

• Using hot water (sets the stain)
• Scrubbing the stain
• Using scented sprays before deodorizing

How to Remove Dried or Set-In Urine Stains and Odors

Old urine stains are more stubborn because they’ve had time to sink deep into your mattress fibers, creating both visible marks and persistent smells. But don’t worry—it’s still possible to clean them effectively using the right method and a little patience.

Step 1: Pre-Treat with Enzymatic Cleaner

• Choose an enzymatic cleaner like Rocco & Roxie or Nature’s Miracle.
• Spray the stained area thoroughly and allow it to sit for 15–30 minutes.
• These products break down the uric acid crystals that cause odor.

Step 2: Gently Blot the Area

• After soaking, use paper towels or a cloth to blot the area.
• Repeat as needed until the surface feels just slightly damp.

Step 3: Apply a Hydrogen Peroxide Paste (Optional for Stains)

• Mix:
  • 8 oz hydrogen peroxide (3%)
  • 3 tablespoons baking soda
  • A few drops of dish soap
• Apply the paste to the stain using a soft brush or sponge.
• Let it sit until fully dry (usually a few hours), then vacuum off the residue.

Step 4: Deodorize with Baking Soda

• Sprinkle a heavy layer of baking soda over the cleaned area.
• Let it sit for 8–12 hours to absorb any lingering smells.
• Vacuum thoroughly.

Step 5: Repeat if Necessary

• For deeply embedded odors, repeat the enzymatic treatment after 24 hours.
• Dried urine may take two or more treatments to fully resolve.

How to Protect Your Mattress from Future Urine Accidents

Once you’ve dealt with the unpleasant task of removing urine from a mattress, the last thing you want is to go through it again. Prevention is not only possible but easy with the right tools and habits.

Use a Waterproof Mattress Protector

• A high-quality protector creates a barrier between spills and your mattress.
• Choose a breathable, hypoallergenic version to maintain comfort.
• Recommended: SafeRest Premium Waterproof Mattress Protector
  • Soft cotton surface, noiseless, and machine washable.

Consider Disposable Bed Pads (for Elderly or Potty Training)

• Ideal for overnight protection during potty training or elder care.
• Easy to change and dispose of after accidents.
• Look for non-slip, odor-locking pads like Medline or Prevail brands.

Set Up a Mattress Cleaning Routine

• Vacuum your mattress monthly to remove dust and debris.
• Deodorize naturally by sprinkling baking soda before vacuuming.
• Use a UV sanitizing light if available, especially in humid climates.

Limit Drinks Before Bed

• Encourage kids or elderly family members to avoid heavy fluids at least 1–2 hours before bedtime.
• Install night lights to reduce bathroom accidents caused by disorientation.

Pet Training and Behavior Tips

• If you have pets, make sure they are house-trained or crate-trained at night.
• Consider pet deterrent sprays to keep them off the bed.

What to Do When the Smell Still Won’t Go Away

Sometimes, even after a deep clean, that unmistakable urine smell seems to return. This happens because urine crystals can remain deep within the mattress fibers, especially if the stain was set in. Here’s how to tackle the toughest odors once and for all.

Reapply an Enzymatic Cleaner

• Enzyme cleaners don’t mask odors—they break down the uric acid that causes them.
• For old or stubborn smells, apply generously and cover the area with plastic wrap to prevent evaporation.
• Let it sit for 12–24 hours, then blot and air dry.
• Repeat 2–3 times if needed.

Use Activated Charcoal or Odor-Absorbing Packs

• Place activated charcoal sachets or bamboo charcoal bags under your mattress or in pillowcases.
• These absorb odors without masking them with fragrance.
• Change or recharge them every few weeks for lasting results.

Deep Clean with a Steam Cleaner

• A steam cleaner penetrates deep layers, lifting out bacteria and odor-causing residues.
• Ensure the mattress is dried thoroughly afterward to avoid mildew.

Use White Vinegar and Baking Soda Again

• Sometimes a second round of the vinegar + baking soda method (covered earlier) can reach deeper spots.
• Always vacuum after the baking soda dries completely.

Air Out the Mattress in Direct Sunlight

• UV rays are a natural disinfectant.
• Placing your mattress outside (or by a sunny window) for 4–6 hours can help break down odor molecules.

When to Replace Your Mattress After a Urine Accident

While most urine stains and smells can be successfully removed, there are situations where replacement is the healthier—and more cost-effective—option. Here’s how to know when it’s time to say goodbye to your old mattress.

The Smell Persists After Multiple Cleanings

• If you’ve:
  • Used enzymatic cleaners multiple times
  • Deep cleaned with steam or baking soda
  • Aired it out and deodorized—and the odor still lingers…
• The urine may have permanently soaked into the inner foam or coils.
• Persistent odor can indicate bacterial growth, which may pose health risks over time.

Visible Staining That Won’t Go Away

• Even after effective odor removal, dark or yellow stains may remain.
• If the stains are large, deep, or located near multiple areas, it may affect the comfort and aesthetic value of the mattress.

 Mold or Mildew Development

• If the mattress was oversaturated during cleaning and didn’t dry properly, mold or mildew can form inside.
• Signs to watch for:
  • A musty smell
  • Allergies or breathing issues while sleeping
  • Visible black or green spots on the surface or edges

Mattress Age and Hygiene Concerns

• If your mattress is 7–10+ years old, replacing it after a serious urine incident may be the wisest choice.
• Old mattresses collect dust mites, sweat, skin cells, and now potentially urine. A new mattress improves overall sleep hygiene.

You’re Just Not Sleeping Well Anymore

• Even after cleaning, psychological discomfort from the accident may affect your rest.
• If you’re sleeping poorly, waking up congested, or noticing aches, that’s a sign it’s time to upgrade.

Conclusion

Urine stains and odors on a mattress can feel overwhelming—but with the right steps, products, and a little persistence, you can restore your bed to a clean and fresh-smelling state. Whether you’re dealing with a fresh spill or an old, stubborn stain, the solutions we’ve shared are effective, safe, and proven to work.

Here’s what to remember:

• Act fast on fresh stains to prevent deep soaking.
• Use enzymatic cleaners to break down odor-causing urine crystals.
• Baking soda and vinegar are powerful, affordable odor-fighters.
• Protect your mattress with waterproof covers and routine cleaning.
• Know when to replace your mattress if hygiene or comfort is compromised.

Frequently Asked Questions About Removing Urine Stains and Smell from a Mattress

Can I use bleach to clean urine from a mattress?

• Not recommended.
  Bleach can:
  • Damage the fabric and foam of the mattress
  • Leave behind a strong chemical smell
  • Be unsafe for pets and kids
    Instead, use enzymatic cleaners specifically designed to break down urine.

How long does it take for urine smell to go away after cleaning?

• It depends on the severity:
  • Fresh stains: Often within 24–48 hours
  • Old or set-in stains: May require multiple treatments over a few days
    Proper drying and airing out are essential to fully eliminate odor.

Is baking soda alone enough to remove urine odor?

• Baking soda is great at absorbing odor, but:
  • It doesn’t break down urine molecules on its own
  • For best results, pair it with vinegar or an enzymatic cleaner
  • Use it after cleaning to neutralize any lingering smell

What’s the best way to dry a mattress after cleaning?

• Speed drying is critical to avoid mold and mildew:
  • Use fans or a blow dryer on cool setting
  • Place mattress in a sunny, well-ventilated room
  • Avoid laying sheets or covers until it’s 100% dry

Can urine damage a memory foam mattress?

• Yes, urine can:
  • Break down foam materials over time
  • Cause permanent odor if not cleaned promptly
  • Lead to bacterial or mold growth in uncleaned areas
    Always act quickly and use cleaners safe for foam.

How do I remove urine smell if I don’t know where it is?

• Try these:
  • Use a blacklight (UV flashlight) to spot hidden stains
  • Sniff around seams and corners—they tend to trap moisture
  • Apply baking soda in sections to isolate smell zones